ChIP-seq analysis of control and DOT1L HET cortical nuclei.

We aimed to understand the changes in H3K79me2 deposition upon monoallelic DOT1L loss in cortical nuclei from 10 week old mice. Overall design: Control and DOT1L HET cortical nuclei were isolated from 10-week-old mice. DOT1L HET mice were heterozygous for floxed DOT1L and both Controls and DOT1L HET were heterozygous for CMV-cre (Strain #:006054). Cortical nuclei were isolated and prepared using the TruSeq ChIP Library Preparation Kit. (n = 4 biological replicates per genotype, 2 male mice and 2 female mice)