Effects of management on soil bacterial and fungal diversity (Shannon Index) of the upper soil layer in nine German vineyards in 2016

Nine experimental vineyards were sampled in the Rhein-Hesse area, Germany, in June 2016. Each vineyard exhibited four experimental plots with different soil management types (complete cover, herbal seed mixture cover, alternating tillage, and complete tillage).
In two inter-rows of each vineyard, a pooled sample of eight soil cores of the upper soil layer (0-10 cm) was used for DNA extraction. The samples were analyzed using Illumina Sequencing by an external sequencing company. The resulting data were further processed to obtain a matrix of OTU and read counts for soil bacteria and soil fungi. Shannon Indices were calculated from these matrixes as metrics of bacterial and fungal diversity.