Effects of management on soil bacterial and fungal diversity (Shannon Index) of the upper soil layer in nine German vineyards in 2016

Nine experimental vineyards were sampled in the Rhein-Hesse area, Germany, in June 2016. Each vineyard exhibited four experimental plots with different soil management types (complete cover, herbal seed mixture cover, alternating tillage, and complete tillage). In two inter-rows of each vineyard, a pooled sample of eight soil cores of the upper soil layer (0-10 cm) was used for DNA extraction. The samples were analyzed using Illumina Sequencing by an external sequencing company. The resulting data were further processed to obtain a matrix of OTU and read counts for soil bacteria and soil fungi. Shannon Indices were calculated from these matrixes as metrics of bacterial and fungal diversity.2016年6月，研究人员在德国莱茵黑森（Rhein-Hesse）区域对9座实验葡萄园开展采样工作。每座葡萄园均设置4个采用不同土壤管理模式的实验样地，分别为完整地表覆盖、草本种子混合覆盖、交替耕作与全耕作。 在每座葡萄园的两条土壤行间内，采集8个表层土壤（0~10 cm）的土芯混合为一份样本，用于DNA提取。随后由外部测序公司采用Illumina测序技术对样本进行分析。所得数据经进一步处理后，得到土壤细菌与土壤真菌的操作分类单元（Operational Taxonomic Unit，OTU）及读段计数矩阵。基于上述矩阵计算香农指数（Shannon Indices），以此作为评估细菌与真菌多样性的指标。