Non-Hybrid, Finished Microbial Genome Assemblies from Long-Read SMRT Sequencing Data

The datasets were collected for demonstrating a hierarchical genome assembly process (HGAP) to generate high quality de novo microbial genome assemblies. The new method utilizes a single, long insert shotgun DNA library in conjunction with Single Molecule, Real Time (SMRT) DNA sequencing and obviates the need for additional sample preparation and sequencing data sets required for previously described hybrid assembly strategies. We demonstrate the efficient de novo assembly from genomic DNA to a finished genome sequence for several microorganisms.