Transitions in lineage specification and gene regulatory networks in hematopoietic stem/progenitor cells over human development

We developed a novel scRNAseq analysis pipeline called SingCellaR. This tool provides a comprehensive set of the functionalities necessary for analysis of large-scale single cell datasets generated by different single-cell technologies. The utility of SingCellaR is illustrated by analysis of a unique dataset capturing hematopoietic stem/progenitor cells (HSPC) across different stages of human ontogeny, from early fetal liver to adult bone marrow, identifying developmental stage- and site-specific differences in the HSPC compartment. We used 10x genomics platform to profile single-cell transcriptome of hematopoietic stem/progenitor cells (HSPCs) sampled throughout the course of human ontogeny. This included early FL (eFL) from first trimester fetuses, paired fetal bone marrow (FBM) and FL from the same second trimester fetuses, paediatric BM (PBM), and adult BM (ABM).