Classifying polycyclic aromatic hydrocarbons by carcinogenic potency using in vitro biosignatures

In this study, we compare genomic signature safter treatment ofprimary human bronchial epithelial cells (HBEC) cultured in 3D with polycyclic aromatic hydrocarbons (PAHs) and identify genesets predictive of cancer risk. Fully differentiated bronchial epithelial cells (n=4 replicates) were treated with 10 and 100 µg/ml benzo[a]pyrene (BAP), 500 ug/ml benzo[a]anthracene (BAN), 100 ug/ml phenanthrene (PHE), 1500 ug/ml coal tar extract, 250 ug/ml simulated air mixture (AM) or acetone vehicle control for 48 hrs and isolated mRNA were sequenced on Illumina HiSeq 3000.