RNA seq, to define the gene expression related to cell wall modification

To understand the genes involved in early embryo development and each genes expression level, transcriptomic analysis was performed at different developmental stages ( 3 h AF: time of increased wall thickness, 10 h AF: time of initiation of rhizoid germination, and 24 h: time that the second cytokinesis finished and the rhizoid elongated). The candidate genes were predicted using the published complete Ectocarpus genome data. Then differential expressed gene were detected in 3 h vs. 10 h, 10 h vs. 24 h, 3 h vs. 24 h. Futhermore, we investigated genes expression involved in alginate synthesis and the architecture of domain.