Investigating chromatin accessibility differences in stem cells and inflammatory cells in heterotopic ossification using ATAC-Seq

Mice were injured and their response in Mesenchymal Stem Cells (MSC) were studied over a period of seven days. The injury site was either non-immobilized or immobilized. The aim of the project is to study how immobilization affects stemness and inflammatory cell response after injury by investigating chromatin accessibility differences using ATAC-Seq. ATAC-Seq libraries were prepared at the UM Epigenomics Core by using the Omni-ATAC protocol for cells as described in Corces et al., 2017 without modifications. ATAC-Seq analysis was carried out on all these samples.