Intra-host genetic diversity analysis of CHIKV populations deciphered from 10 sera of CHIKV+ve patients.

The percentage of variable nucleotide (nt) sites was calculated as the number of variable nt sites ×100 divided by the number of nt analysed (758 nt).
The percentage of nucleotide (nt) mutations was calculated as the number of nt mutations ×100 divided by the number of nt sequenced for each serum sample.
The average pairwise distance was calculated among the nucleotide (p nt) and amino acid (p aa) sequences from each serum.
The mean ratios of non-synonymous (dN) and synonymous (dS) substitutions per site were estimated using the pairwise method of Nei and Gojobori.
Sex: Male: male; F: female.
NA: not available.