Transcriptome Analysis of 3-nitropropanoic acid Effects on COV434 cell lines

Oxidative stress (OS) is regarded as one of the culprits of ovarian dysfunction. OS causes damage to various types of ovarian cells including granulosa cells (GCs), jeopardizing the ovarian microenvironment, disturbing follicular development and participating in various female reproductive disorder. However, the specific molecular pathological mechanisms underlying this process have not been fully elucidated. Therefore, in this study, human ovarian granulosa cell lines COV434 were treated with 3-nitropropanoic acid, a mitochondrial toxin inducing OS by irreversibly inhibiting the succinate dehydrogenase enzyme in the complex II of electron transport chain, to explore the transcriptome changes upon OS exposure. Overall design: Comparative gene expression profiling analysis of RNA-seq data for the control (ctr, in duplicate) group and the 3-nitropropanoic acid (3np, in triplicate) group of COV434 cells.