Research on the Effects of Angelica Dahurica Radix on Migraine Gut Microbiota1

The study protocol received ethical approval (2019-08) from Jthe Ethics Committee of Chengdu University of Traditional Chinese Medicine, with all procedures conducted in strict accordance with established animal welfare standards. Specific-pathogen-free (SPF)-grade adult male Wistar rats (190±20 g) were obtained from Chengdu Dasuo Experimental Animal Co., Ltd (License No.: SCXK (Sichuan) 2020-0030), and were maintained at 22 ± 2 ℃ with a 12 h light/dark cycle with ad-libitum access to food and water in the animal facility. After one week acclimation period, the rats were randomly assigned to four groups (randomized using a computer-generated sequence): Control group (Normal), Model group (Model), BZ group (BZ) and positive drug group (Sumaputan succinate injection, SS). The Normal and Model groups received intragastric administration of sterile water (10 ml/kg) for 14 days, while the BZ group was administered BZ (7.2 g crude drug/kg) orally for 14 consecutive days. The Normal, Model, SS and BZ groups were subcutaneously injected with NTG (10 mg/kg, Beijing Yimin Pharmaceutical Co., Ltd., Beijing, China) every other day for 9 days (5 times in total)