Zea mays subsp. mays Targeted Locus (Loci)

We assessed the performance of a solution-based hybridization method to measure DNA methylation at targeted genomic loci. We found that >25% of the sequencing reads were from the targeted regions, indicating that this method is very efficient for target enrichment. This method is highly reproducible (r is between 0.93 and 0.99) in terms of both read coverage and methylation levels between technical replicates. DNA methylation level assayed using this method is highly correlated with the one that is measured using whole-genome bisulfite sequencing. In addition, we showed that this method can be efficient to assay allele-specific methylation pattern in heterozygous individuals.