Role of DPYSL2A in KLF4-mediated monocytic differentiation in AML cells. Homo sapiens

To compare the global gene expressions in between KLF4- and DPYSL2A-expressed AML cells, we conducted gene expression arrays in THP-1 cells lentivirally-transduced with doxycycline-inducible KLF4 or DPYSL2A. We identified the pivotal role of KLF4-DPYSL2A axis in differentiating AML cells, which could be theraputically-tageted in conventional therapy-resistant AML patients. Overall design: THP-1 cells were transduced with lentivirus encoding KLF4 or DPYSL2A under doxycycline responsive promoters. Cells were treated with 3 μM doxyxicline for 48 hours to induce gene expressions.