Investigation of reversible histone acetylation and dynamics in gene expression regulation using 3D liver spheroid model. Investigation of reversible histone acetylation and dynamics in gene expression regulation using 3D liver spheroid model

To investigate epigenetic dynamics in a 3D liver spheroid model, liver spheroids were treated with sodium butyrate for 3 days and allowed to recover for 1 week. We then performed gene expression profiling analysis using data obtained from RNA-seq of spheroids at 4 time points Overall design: Comparative gene expression profiling analysis of RNA-seq data for HepG2 liver spheroids