CARM1/PRMT4 is essential for myeloid leukemogenesis but dispensable for normal hematopoiesis

We investigated the role of PRMT4 in normal and malignant hematopoiesis. We found that knockdown of PRMT4 impairs cell cycle progression, induces apoptosis, and downgretulateds E2F target genes in leukemia cell lines, identifying several mechanisms for the leukemia-specific dependence on PRMT4. Three AML cell lines (SKNO1, MV411, and MOLM-13) were subjected to high-throughput RNA sequencing three days after shRNA inhibition of PRMT4 or scramble control. All cell lines sequencing experiments were repeated in triplicate. Differential expression of each cell line was performed independently comparing to controls. Additionally, differential analysis was performed by combining all cell line PRMT4 inhibited cells and comparing to all controls.