mRNA expression in white adipose tissue of adult F2 female offspring from F0-fathers fed a chow or high-fat diet. Rattus norvegicus

The purpose of this study was to investigate whether paternal high-fat diet (HFD) transgenerationally remodels the epigenome of spermatozoa to alter metabolism in the F1 and F2 generation offspring White adipose tissue mRNA expression profiling of F2-female offspring from F0-founders fed either a chow or a chronic HFD challenged. Adult females were challenged or not a high-fat diet for 12 weeks. White adipose tissue was dissected at an endpoint experiment. Rats were subjected to 4 hours fasting prior to anesthesia with pentobarbital and tissue collection. Overall design: F2-females Sprague-Dawley rats from F0 founders fed or not a high-fat diet were also subjeted to high-fat consumption for 12 weeks. At terminal experiment, gonadal white adipose from 6 to 7 animals per group was collected, prepared and subjeted to the array analysis.