File S1 - Enriched Environment Prevents Hypobaric Hypoxia Induced Memory Impairment and Neurodegeneration: Role of BDNF/PI3K/GSK3β Pathway Coupled with CREB Activation

Knockdown of TrkA and TrkB with antisense techniques shows promising inhibition of expression of their respective receptors as shown in Figure S1. Figure S2 describes TrkB mediated activation of ERK and PI3K pathways. Western blot analysis showed that inhibition of ERK pathway with U0216 and PI3K pathway with Wortmannin decreased the expression of pERK (A) and pAKT (B) respectively without altering their basal level. Interestingly it has been observed that knocking down the TrkB receptor fails to phosphorylate GSK3b and hence inactivation even in presence of enriched environment as shown in Figure S3. This shows that in present case BDNF mediated TrkB activation is necessary event for GSK3b inactivation. Data represents Mean ± SEM. “*” and “#” represents p**” represents p (DOC)