Homo sapiens. Homo sapiens

NIAID Data Ecosystem2026-03-09 收录

RNA测序文库构建

基因组测序方法开发

数据链接：

https://www.ncbi.nlm.nih.gov/bioproject/PRJNA314737 数据链接链接失效反馈

官方服务：

资源简介：

Proof-of-principle sequencing runs for the Capture and Amplification by Tailing and Switching (CATS) library generation method development

创建时间：

2016-03-09

Transposase assisted tagmentation of RNA/DNA hybrid duplexes. Transposase assisted tagmentation of RNA/DNA hybrid duplexes

RNA测序文库构建

RNA-DNA杂交体分析

Tn5-mediated transposition of double-strand DNA has been widely utilized in various high-throughput sequencing applications. Here, we report that the Tn5 transposase is also capable of direct tagmenta

NIAID Data Ecosystem70

Depletion of erythropoietic miR-486-5p and miR-451a improves detectability of rare microRNAs in peripheral blood-derived small RNA sequencing libraries

小核酸分析

RNA测序文库构建

Erythroid-specific miR-451a and miR-486-5p are two dominant microRNAs (miRNAs) in human peripheral blood-derived small RNA sequencing libraries. Their overabundance reduces diversity as well as comple

NIAID Data Ecosystem60

library construction protocol for ultralow RNA-seq method

RNA测序文库构建

低丰度基因检测

We present a highly sensitive library construction protocol suitable for subcellular or ultralow input RNA sequencing (ulRNA-seq). In order to improve the sensitivity and low abundance gene detection

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Additional file 12: Table S2. of Systematic comparison of small RNA library preparation protocols for next-generation sequencing

RNA测序文库构建

实验寡核苷酸设计

Oligonucleotides used in this study. (XLSX 9 kb)

Figshare2018-02-06 更新60

Sequencing libraries constructed for this study.

RNA测序文库构建

高通量测序

Details of barcodes, adaptors and samples used for constructing RNA, DNA, and SMRT Sequencing libraries. (XLSX)

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