Yarrowia deformans strain CBS 2071 genome sequence. Yarrowia deformans strain CBS 2071 genome sequence

The genome of Yarrowia deformans strain CBS 2071 has been sequenced using Illumina Solexa technology with a shot-gun library and a mate pair library of 8 kb in average. The raw reads were trimmed with Trimmomatic and cutadapt. The assembly was done using SOAPdenovo2 with a kmer of 73. Two successive runs of GapCloser were used to close gaps. The current draft genome sequence is composed of 43 scaffolds larger than 5 kb (41 nuclear and 2 mitochondrial ones), for a total size of the nuclear DNA of 21,059,484 bp with an N50 of 1,302,329 bp (6 scaffolds) and a G+C content of 50.3%. Annotation of protein-coding genes was performed using the Amadea Annotation transfer tool (Isoft, France), with Yarrowia lipolytica genome as reference. The structural annotation of these genes was manually curated using RNA-Seq data and other genetic elements (tRNA and transposable elements) were expertly added.