Data for: RNA-Seq approach for accurate characterization of splicing efficiency of yeast introns
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The data file has 7 columns and 305 rows containing information for 304 yeast introns, including 24 introns located in 5' UTR: 1. Gene: list the gene name containing individual introns. The first 24 introns are located in 5' UTR of 24 genes. 2. N_EE: number of transcriptomic reads mapped to exon-exon junction sequences 3. N_EI5: number of transcriptomic reads mapped to exon-intron junction sequences at the 5' end 4. N_EI3: number of transcriptomic reads mapped to exon-intron junction sequences at the 3' end 5. L_intron: intron length 6. p_EI5: estimated proportion of EI5 according to Eq. (1) of the manuscript. 7. SE: Splicing efficiency The transcriptomic data are from four files with SRA accessions SRR3440202, SRR3440204, SRR3440206, SRR3440207. They are from BioProject (project accession PRJNA319604). They are the first four of a total of 44 files.
本数据集文件包含7列、305行数据,涵盖304个酵母内含子的相关信息,其中包含24个定位于5'非翻译区(5' UTR)的内含子。各字段详情如下:1. Gene:标注包含单个内含子的基因名称,前24个内含子分别位于24个基因的5' UTR区域。2. N_EE:比对至外显子-外显子连接序列的转录组读段数量。3. N_EI5:比对至5'端外显子-内含子连接序列的转录组读段数量。4. N_EI3:比对至3'端外显子-内含子连接序列的转录组读段数量。5. L_intron:内含子长度。6. p_EI5:依据本文稿公式(1)估算得到的EI5占比。7. SE:剪接效率(Splicing efficiency)。该转录组数据源自4个序列读段存档(Sequence Read Archive, SRA)文件,其SRA登录号分别为SRR3440202、SRR3440204、SRR3440206、SRR3440207,所属生物项目(BioProject)的登录号为PRJNA319604,为总计44个文件中的前4个。




