Single cell RNA-sequencing reveals cellular heterogeneity and trajectories of lineage specification

In order to study the regulatory mechanism of stem cell therapy for sarcopenia and the influence of related genes on sarcopenia Overall design: The coordinated spatial and temporal regulation of gene expression in the murine hindlimb determines the identity of mesenchymal progenitors and the development of diversity of musculoskeletal tissues they form. Hindlimb development has historically been studied with lineage tracing of individual genes selected a priori, or at the bulk tissue level, which does not allow for the determination of single cell transcriptional programs yielding mature cell types and tissues. In order to study the regulatory mechanism of stem cell therapy for sarcopenia and the influence of related genes on sarcopeniawe used single cell mRNA sequencing (scRNA-seq) to profile the developing murine hindlimb.