Single cell transcriptomics of blood mononuclear cells in patients with preeclampsia

Study of single cell transcriptomics of PBMC from patients with preeclampsia and normal pregnancy Overall design: The study compared changes occurring at the transcriptome level in umbilical cord blood PBM? during normal pregnancy and preeclampsia. During a cesarean section, after the placenta was removed, the umbilical vessel was punctured and blood was collected into EDTA tubes. Isolation of PBMC was carried out in a Ficoll density gradient for 20 min at 800 g with break 0. The PBM? ring was rinsed in PBS and frozen. The samples were then analyzed using single cell transcriptomic analysis