Amorphous calcium phosphate-coated surfaces as a model for bone microenvironment in prostate cancer.

To develop a model for bioavailable calcium, usable for bone metastasis studies. The present study focuses on the effect of a calcium-rich surface on prostate cancer. Overall design: Comparative gene expression profiling analysis of RNA-seq data from non tumorogenic prostate epithelial cells (RWPE), early adenocarcinoma model cells (LNCaP) and Bone metastatic cells (VCaP, MDAPCa2a), cultured on calcium and normal plastic culture surfaces, in both the presence and absence of testosterone