Elemental biomass composition (bold) estimated from overall chemical reactions for growth on acetate under varying levels of DNA:RNA:protein (P):fatty acid (FA).

Estimates were obtained from simulations under redox conditions corresponding to the concentrations of NADP+:NADPH being held at the thermodynamic odds (Eq 24) of 107. The rows labeled with DNA:RNA:protein:lipid values are those from the optimization for growth of the metabolic model while those labeled estimate are estimates of the biomass elemental composition using the stoichiometry found in the model, and can be compared to the similar condition shown for acetate growth shown in Table 7 in which the respective levels were 1.0: 2.9: 44.1: 8.5. The grey rows are for those compounds in the overall chemical equation for the metabolic model that are not used in estimating the elemental composition of the biomass. Abbreviations: ABP: adenine-3,5-bisphosphate; THF: tetrahydrofolate; 5,10-MTHF: 5, 10 methylenetetrahydrofolate; N10-fTHF: N10-formyltetrahydrofolate; Pi,2: diphosphate.