Proteome data of Amphistegina gibbosa

The proliferation of key marine ecological engineers and carbonate producers often relies on their association with photosymbiotic algae. Evaluating stress responses of these organisms is important to predict their fate under future climate projections. Physiological approaches are limited in their ability to resolve the involved molecular mechanisms and attribute stress effects to the host or symbiont, while probing and partitioning of proteins cannot be applied in organisms where the host and symbiont are small and cannot be physically separated. Here we apply a label-free quantitative proteomics approach to detect changes of proteome composition in the diatom-bearing benthic foraminifera Amphistegina gibbosa experimentally exposed to three thermal-stress scenarios. We developed a workflow for protein extraction from less than ten specimens and simultaneously analysed host and symbiont proteomes. […]

关键海洋生态工程师和碳酸盐生产者的广泛分布，往往依赖于其与光合共生藻类的关联。评估这些生物的胁迫响应对于预测未来气候变化情景下它们的命运至关重要。生理学方法在解析涉及的分子机制和将胁迫效应归因于宿主或共生体方面能力有限，而在宿主和共生体体积微小且无法物理分离的生物中，蛋白质的探测和分配方法亦难以应用。本研究采用了一种无需标记的定量蛋白质组学方法，以检测实验中暴露于三种热胁迫情景下的有孔虫Amphistegina gibbosa所携带的硅藻中蛋白质组组成的改变。我们开发了一套从不到十个样本中提取蛋白质的流程，并同时对宿主和共生体的蛋白质组进行了分析。...