<i>de novo</i> heterochromatin domains are associated with reduced gene expression at both the <i>ura4</i> and <i>spbc2f12.03</i> loci.

1From Figure 1A and 1C. 2Distance of the translation start site from the nearest edge of L5. 3Number of independent RNA isolations included in analysis. 4p-value resulting from comparison between ura4::ade6+ and ura4::L5-ade6+ strains. 5Essential for viability [82]. 6Mutation in mlo3 results in a growth defect [83]. 7Essential for viability [84]. Relative gene expression in the ura4 and spbc2f12.03 loci. Steady-state mRNA for genes in the ura4 (A–H) and spbc2f12.03 (1–7) loci were assayed via quantitative RT–PCR and are reported relative to ura4::ade6+ control strains. The distance from the transcription start site to the nearest edge of L5 is indicated for each gene.