Data_Sheet_1_Distribution of GABAergic Neurons and VGluT1 and VGAT Immunoreactive Boutons in the Ferret (Mustela putorius) Piriform Cortex and Endopiriform Nucleus. Comparison With Visual Areas 17, 18 and 19.zip

We studied the cellular organization of the piriform network [comprising the piriform cortex (PC) and endopiriform nucleus (EP)] of the ferret (Mustela putorius)—a highly excitable region prone to seizures—and, more specifically, the distribution and morphology of different types of gamma-aminobutyric acid (GABA)ergic neurons, and the distribution and ratio of glutamatergic and GABAergic boutons, and we compared our findings to those in primary visual area 17, and secondary areas 18 and 19. We accomplished this by using cytochrome oxidase and immunohistochemistry for mature neuronal nuclei (NeuN), GABAergic neurons [glutamic acid decarboxylase-67 (GAD67), calretinin (CR) and parvalbumin (PV)], and for excitatory (vesicular glutamate transporter 1; VGluT1) and inhibitory (vesicular GABA transporter; VGAT) boutons. In the ferret, the cellular organization of the piriform network is similar to that described in other species such as cats, rats and opossums although some differences also exist. GABAergic immunolabeling showed similarities between cortical layers I–III of the PC and visual areas, such as the relative distribution of GABAergic neurons and the density and area of VGluT1- and VGAT-immunoreactive boutons. However, multiple differences between the piriform network and visual areas (layers I–VI) were found, such as the percentage of GABAergic neurons with respect to the total number of neurons and the ratio of VGluT1- and VGAT-immunoreactive boutons. These findings are relevant to better understand the high excitability of the piriform network.

本研究聚焦于研究沙鼠（Mustela putorius）梨状网络（由梨状皮层（PC）和内梨状核（EP）组成）的细胞组织结构——这一区域具有高度兴奋性且易发癫痫——并进一步探讨了不同类型γ-氨基丁酸（GABA）能神经元的分布和形态学特征，以及谷氨酸能和GABA能突触的分布和比例。我们的研究结果与初级视觉区17以及次级区域18和19进行了比较。本研究通过使用细胞色素氧化酶和免疫组化技术对成熟神经元核（NeuN）、GABA能神经元[谷氨酸脱羧酶-67（GAD67）、钙调蛋白（CR）和副白蛋白（PV）]以及兴奋性（囊泡谷氨酸转运蛋白1；VGluT1）和抑制性（囊泡GABA转运蛋白；VGAT）突触进行了分析。在沙鼠中，梨状网络的细胞组织结构与猫、鼠和负鼠等物种的描述相似，尽管也存在一些差异。GABA能免疫染色揭示了梨状皮层第I至III层与视觉区域之间的相似性，例如GABA能神经元的相对分布以及VGluT1和VGAT免疫反应突触的密度和面积。然而，梨状网络与视觉区域（第I至VI层）之间也存在多个差异，如GABA能神经元占总神经元数的百分比以及VGluT1和VGAT免疫反应突触的比例。这些发现有助于更深入地理解梨状网络的高兴奋性。