Transcriptome profiling of inflorescence stem tissues from Arabidopsis thaliana series 1

We report the Fluorescence Activated Nucleus Sorting (FANS)-based RNA sequencing for high resolution transcriptome profiling of the second internode of the Arabidopsis thaliana inflorescence stem. We compared GFP-positive nuclei and GFP negative nuclei from promoter reporter lines expressing Histone 4-GFP (NST3pro:H4-GFP, SMXL5pro:H4-GFP, APLpro:H4-GFP), and compared GFP positive nuclei from seven lines expressing Histone 4-GFP under the control of different tissue-specific promoters (NST3pro:H4-GFP, VND7pro:H4-GFP, PXYpro:H4-GFP, SMXL5pro:H4-GFP, APLpro:H4-GFP, SCRpro:H4-GFP, LTP1pro:H4-GFP). mRNA profiles of GFP-positive and GFP-negative nuclei from inflorescence stems of Arabidopsis thaliana NST3pro:H4-GFP, SMXL5pro:H4-GFP, APLpro:H4-GFP transgenic lines, respectively, were compared. In addition, mRNA profiles of GFP-positive nuclei from inflorescence stems of Arabidopsis thaliana of NST3pro:H4-GFP, VND7pro:H4-GFP, PXYpro:H4-GFP, SMXL5pro:H4-GFP, APLpro:H4-GFP, SCRpro:H4-GFP, LTP1pro:H4-GFP transgenic lines were also compared. 3 replicates for each sample were provided.