Data for: Refractive index-assisted UV/Vis spectrophotometry to overcome spectral interference by impurities: applications from impurity analysis to salinity and protein measurements

It is a study on matrix effects and a new refractometric method that can help analytical techniques like UV/Vis spectrophotometry deal with matrix effects, specifically, spectral interference. Spectral interference due to matrix impurities is a primary reason that hinders the large-scale application of simple analytical techniques like UV/Vis spectrophotometry in complex samples like those in food, beverage, environmental and other industries. There are lengthy studies on the effects of possible interferents in specific cases. However, current approaches to ensure the absence of huge spectral interference errors are either slow or costly unless all possible impurities are known. We have introduced a simple, cost-effective, and rapid, refractive index-based technique which can assist UV/Vis spectrophotometry for detecting and reducing spectral interference in many of these cases. We verified it in more than ten solvents. We also performed more studies on its application in impurity analysis and its capability in detecting interference errors in the standard UV spectrophotometric methods for protein concentration measurement and salinity determination. We hope that the results would encourage the use of this new technique in certain branches of analytical chemistry. This dataset contains the data which was used for the figures and discussion of the results in the original manuscript. It includes, among others, 1) The UV spectra and refractometric measurements of benzene and N, N-Dimethylaniline in cyclohexane 2) Lorentz Lorenz mixing rule applied to refractometric measurements of benzene in ten different solvents (acetonitrile, n-butanol, cyclohexane, CCl4, p-xylene, toluene, o-xylene, chlorobenzene, N, N-Dimethylaniline and aniline) 3) Qualitative analysis of impurity compounds using Pearson's correlation coefficient, refractometry and UV spectrophotometry 4) The refractometric and UV spectrophotometric standard curves of protein and artificial seawater. Interference effects of DNA, RNA, lysozyme and Triton X-100 on protein (BSA) analysis and nitrates and nitrites on salinity measurement are included in the original paper.本研究围绕基质效应展开，并提出一种新型折光分析法，可辅助紫外-可见分光光度法（UV/Vis spectrophotometry）等分析技术解决基质效应问题，尤其是光谱干扰问题。由基质杂质引发的光谱干扰，是制约紫外-可见分光光度法等简易分析技术在食品、饮料、环境等行业复杂样品中大规模应用的主要因素。目前已有针对特定场景下潜在干扰物影响的大量研究，但当前保障光谱干扰误差可控的方法，要么耗时较长，要么成本高昂，除非已知所有可能存在的杂质。本研究提出了一种基于折光率的简易、高性价比且快速的分析技术，可在多数此类场景中辅助紫外-可见分光光度法检测并削弱光谱干扰。我们已在十余种溶剂中验证了该技术的有效性，并进一步开展了相关研究：探索其在杂质分析中的应用，以及其在蛋白质浓度测定、盐度测定的标准紫外分光光度法中检测干扰误差的能力。本研究期望该技术的研究成果能够推动其在分析化学若干分支领域中的应用。本数据集包含了原始手稿中用于结果配图与讨论的全部实验数据，具体涵盖以下内容：1）环己烷中苯与N,N-二甲基苯胺的紫外光谱及折光率测量数据；2）针对苯在十种不同溶剂（乙腈、正丁醇、环己烷、四氯化碳（CCl4）、对二甲苯、甲苯、邻二甲苯、氯苯、N,N-二甲基苯胺与苯胺）中的折光率测量数据所应用的洛伦兹-洛伦兹混合法则（Lorentz-Lorenz mixing rule）相关数据；3）基于皮尔逊相关系数（Pearson's correlation coefficient）、折光法与紫外分光光度法开展的杂质化合物定性分析数据；4）蛋白质与人工海水的折光法及紫外分光光度法标准曲线数据。原文还涉及脱氧核糖核酸（DNA）、核糖核酸（RNA）、溶菌酶与曲拉通X-100（Triton X-100）对蛋白质（牛血清白蛋白，BSA）分析的干扰效应，以及硝酸盐、亚硝酸盐对盐度测定的干扰效应相关内容。