Supporting data for “Identification and characterization of pharmacologically active small molecules modulating spheroid attachment and embryo implantation”

Infertility affects millions of couples worldwide, with embryo implantation failure being one of the biggest challenges. This research aims to identify and characterize small molecules that can enhance endometrial receptivity and embryo implantation. A multi-step approach was used, including high-throughput screening of 1280 small molecules from the Library of Pharmacologically Active Compounds (LOPAC), bioinformatics analysis, and in vitro co-culture assays with non-receptive endometrial cells to identify promising compounds. RNA sequencing and quantitative PCR were applied to assess differential gene expression, focusing on pathways relevant to implantation. In vivo validation was conducted using a transcervical delivery system in a mouse model, with implantation sites marked by Chicago blue dye at specific time points to precisely evaluate implantation outcomes​​​.

不孕症全球范围内影响着数百万对夫妇，其中胚胎着床失败是最大的挑战之一。本研究旨在识别并表征能够增强子宫内膜接受性和胚胎着床的小分子。研究采用了多步骤方法，包括对药理活性化合物库（LOPAC）中1280种小分子的高通量筛选、生物信息学分析以及与非接受性子宫内膜细胞的体外共培养实验，以识别有潜力的化合物。通过RNA测序和定量PCR技术评估了差异基因表达，重点关注与着床相关的通路。在实验室内，使用经宫颈输送系统在鼠模型中进行了体内验证，通过在特定时间点用芝加哥蓝染料标记着床位点，以精确评估着床结果。