Protein lysates were collected from WT or Xrn1 ko A549 cells and HEKT293T ishXrn1 cells treated with doxycycline for 3-4 days or left untreated. They were then probed with antibodies against Xrn1 or b
Western blot on epidermal extracts prepared with urea or SDS. The data show that the case has not the typical bands of PNP and thus the patient is not affected by PNP.
Cells were transfected with siNR4A1 oligonucleotides (A, B) (17) or treated with DIM-C-pPhOH and DIM-C-pPhCO2Me (C, D), and whole cell lysates were analyzed by Western blots as described in the Materi
HeLa cells were infected with wild-type Ad3, Ad14, and Ad5 at an MOI of 500 vp/cell for 7 hours. Thirty-six hours after infection, cell lysates were subjected to ultracentrifugation on a sucrose gradi
