Root transcriptional profile of wheat seedlings in response to Fe, S and dual (Fe and S) deficiencies.

The comparison of transcriptional profiles of roots grown under Fe, S and dual deficiency with those obtained for roots of control plants (grown in a complete nutrient solution) allowed identifying the transcripts differentially expressed in response to each deficiency. Transcriptional profiles were obtained using roots of 5-day-old wheat seedlings grown for 14 additional days in four different nutritional conditions (control, C, 1.2 mM sulfate and 80 µM FeIII-EDTA; Fe deficiency, Fe, 1.2 mM sulfate and 0 µM FeIII-EDTA; S deficiency, S, 0 mM sulfate and 80 µM FeIII-EDTA; dual deficiency, D, 0 mM sulfate and 0 µM FeIII-EDTA).