Additional file 4: Figure S4. of Targeting VEGFR-3/-2 signaling pathways with AD0157: a potential strategy against tumor-associated lymphangiogenesis and lymphatic metastases

Effect of AD0157 on MDA-MB-231/Luc+ cell migration and apoptosis. (a) Closure of the initial scratched area by MDA-MB-231/Luc+ cells after 48 h in the absence or presence of different AD0157 doses. Broken lines in pictures indicate the initial (time 0) wound edges. Scale bars represent 100 μm. (b) Graph shows the percentage of the initial cell-free area recovered by tumor cells. (c) Representative pictures showing the effect of AD0157 on MDA-MB-231/Luc+ cell chromatin condensation after 14 h of treatment. Scale bars represent 100 μm. (d) Percentages of control and AD0157-treated tumor cells with condensed chromatin (total cells were counted by using bright field). (e) Percentages of subG1, G1, and S/G2/M MDA-MB-231/Luc+ cell subpopulations analyzed by flow cytometry after AD0157 treatment. (PS 17413 kb)