Data underlying the research on particle manipulation using hydrodynamic forcing. This dataset consist of the experimental data of an arbitrarily selected pollen grain trapping in a Hele-Shaw cell.

The reseach objective is to present a microfluidic approach to achieve the dynamic control of particle pathlines within a flow through microfluidic device. Our approach is general, and not limited to one particular kind of particle. The flow is used as gentle tweezers to move particles within the flow cell and the approach is particularly suited to biological sample. To demonstrate this, an arbitrary pollen grain is selected and trapped in the flow cell while the other pollen grains continue to flow. Our approach combines three key aspects: the design of a flow-through microfluidic flow cell with the ability to manipulate the streamlines of the flow, an optimization procedure to find a priori optimal particle path-lines, and a Proportion-Integral-Derivative-based (PID) feedback controller to provide real time control over the particle manipulations. The experimental raw images were recorded with a sCMOS camera (PCO) with a pixel pitch of 6.5 μm. The camera was mounted on a microscope (Nikon Eclipse Ti) with a 1x objective. The acquisition frequency was 5 Hz corresponding to an average in-plane displacement of 4-6 pixels between two consecutive recordings. An additional sCMOS camera (PCO) with 3x zoom lens is used for the visualization of the trapped pollen. The zip file contains the raw images and the MATLAB script used to do an experiment of arbitarily selecting a pollen grain and trapping it by only using the hydrodynamic forcing in a Hele-Shaw cell.

本研究旨在提出一种微流控方法，实现微流控装置内流场中粒子运动轨迹的动态调控。该方法具有普适性，不受限于单一类型的粒子。研究将流场作为柔性镊子，用于操控流腔内的粒子，尤其适用于生物样本。为验证该方法的有效性，我们选取任意一颗花粉粒将其捕获于流腔内，其余花粉粒则继续随流运动。本方法包含三大核心环节：一是设计可调控流场流线的通流式微流控流腔；二是构建先验最优粒子运动轨迹的优化流程；三是采用基于比例-积分-微分（Proportion-Integral-Derivative, PID）的反馈控制器，实现粒子操控的实时调控。实验原始图像由像素间距为6.5μm的sCMOS相机（PCO）采集，该相机搭载于配备1×物镜的尼康（Nikon）Eclipse Ti显微镜上。采集频率设置为5赫兹，对应两次连续采集之间的平面内平均位移为4~6个像素。此外，还配备了搭载3×变焦镜头的sCMOS相机（PCO），用于可视化观测被捕获的花粉粒。本压缩文件包含原始图像与MATLAB脚本，可用于开展仅通过流体动力操控在赫勒-肖（Hele-Shaw）流腔内任意选取并捕获花粉粒的实验。