The Heat-Inducible Transcription Factor HsfA2 Enhances Anoxia Tolerance in Arabidopsis

Anoxia induces several heat shock proteins and a heat pre-treatment can acclimatize Arabidopsis seedlings to a subsequent anoxic treatment. In this work we analyzed the response of Arabidopsis seedlings to anoxia, heat and a combined heat+anoxia stress. A significant overlapping between the anoxic and heat shock responses has been observed by whole-genome microarray analysis. Overall design: We treated Arabidopsis seedling, 4-days old, dark germinated with: Control (23C, dark, liquid Murashige-Skoog medium containing 30mM sucrose). Heat-treated (38C for 90 minutes, dark, liquid Murashige-Skoog medium containing 30mM sucrose). Anoxia-treated (23C, under anoxia for 6h, dark, liquid Murashige-Skoog medium containing 30mM sucrose). Combined heat+Anoxia-treatment (23C, treated at 38C for 90 min and thereafter under anoxia for 6h, dark, liquid Murashige-Skoog medium containing 30mM sucrose). Two biological replicates for each condition.