Bulk RNA-seq was performed on murine Lin-Sca+Kit+ (LSK) bone marrow cells from a transgenic SCLtTA/BCR-ABL (DTG) mouse BM cells (CD45.2) in which BCR-ABL1 was expressed or not expressed

Bulk RNA-seq was performed on murine Lin-Sca+Kit+ bone marrow cells from a transgenic SCLtTA/BCR-ABL (DTG) mouse BM cells (CD45.2) to understand the molecular consequences of BCR-ABL1 expression on the global mRNA programme of these cells. Eight to twelve week old B6.SJL recipient mice (CD45.1) were irradiated with two doses of 4.25Gy, 3hrs apart and transplanted with 2 x 106 SCLtTA/BCR-ABL (DTG) mouse BM cells (CD45.2) via tail vein injection. Tetracycline (TET) was included in the drinking water for two weeks post-transplant to allow time for recovery and engraftment. Two weeks post irradiation, TET was removed for two weeks in one cohort, allowing BCR-ABL1 expression and leukaemia development (n=6; CML group), while TET administration was continued In a second cohort (n=6; CTRL group). RNA was extracted and libraries made for RNA-seq analysis. Both male and female age-matched recipient mice were used for this study.