Effects of lipopolysaccharide (LPS) or interferon gamma (IFNy) treatment on wild type or PILRA knock-out iPSC-derived human microglia after 24 hours

To study the effect of PILRA loss-of-function immune signaling pathways, we treated wildtype and PILRA knock-out (KO) iPSC-derived microglia (iMGs) with lipopolysaccharide (LPS) or interferon gamma (IFNy) for 24 hours and performed bulk RNA-seq analysis. Overall design: iPSC-derived microglia (iMG) with either wildtype or PILRA knock-out genotypes were treated with either 1) LPS, 2) IFNg or 3) left untreated for 24 hours. The experiment was repeated five times, starting with freshly seeded iMG on different days.