Global Transcriptome Analysis during Mucociliary Differentiation of Human Nasal Polyp/Nasal Mucosa Basal Stem/Progenitor Cells

The aim of this study was to perform a genome-wide transcriptional analysis (mRNA + microRNA) during in vitro mucociliary differentiation of primary human basal stem/progenitors cells (BSCs) cultured at the air-liquid interface (ALI) system. We used microarrays to detail the global gene expression underlying mucociliary differentiation of human upper airways basal stem/progenitor cells isolated from nasal polyps and control nasal mucosa. Basal stem/progenitor cells were isolated from Nasal Polyps or control Nasal Mucosa by the explant technique, cultured and differentiated at the air-liquid interface (ALI) cell culture system. At the different days of ALI culture (0, 14 and 28 days), total RNA was extracted from cells and finally processed for microarrays analysis (Human Genome U219 24-Array Plate).