Pseudomonas aeruginosa, Echerichia coli, Mycobacterium tuberculosis, and uncultivated soil bacterium Raw sequence reads and de novo assembled draft genomes

We developed a microfluidic device that processes bacterial samples in the order of 10^3 cells or picograms of genomic DNA into whole genome shotgun sequencing libraries. Four different sample types processed with our micro-device and sequenced on the HiSeq 2500 platform (2 x 125 & 2 x 101) are uploaded: 1) 124 50-picogram genomic DNA input clinical isolates of Pseudomonas aeruginosa from six patients collected at Brigham and Women's hospital (Boston, USA), 2) 36 1,000-cell input Escherichia coli BL21-DE3 3) 18 10,000-cell input Mycobacterium tuberculosis OFXR-14 and OFXR-16, and 4) 14 10,000 to 100,000-cells from soil micro-colonies cultured using the iChip. The clinical Pseudomonas samples were discard samples where the connection with the patient metadata has been lost. These samples were collected through four different collection methods (BAL, sputum, urine, thoracostomy). The soil micro-colonies were from a Boston private garden