RNA sequencing of human primary fibroblasts in 3D gelatin-based hydrogels were treated with 200 mM sorbitol for 9 h and 24 h.. undefined

Fibroblasts were embedded in 4% gelatin-methacryloyl (GelMA) hydrogels and left for recovery for 3 days before sorbitol treatment. Experimental groups: 1. Ctrl (medium-treated) 9 h, n=3 2. 200 mM sorbitol 9 h, n=3 3. 200 mM sorbitol 24 h, n=3 For RNA isolation from hydrogels, samples were homogenized in TRIreagent® and RNA was extracted according to the manufacturer’s instructions for tissue extraction.