Polyvinyl alcohol expanded mouse HSC cultures (scRNA-Seq). Polyvinyl alcohol expanded mouse HSC cultures (scRNA-Seq)

We previously reported a polyvinyl alcohol-based mouse hematopoietic stem cell (HSC) culture protocol that efficiently expanded transplantable HSCs for at least a month ex vivo (Wilkinson et al., Nature 2019). Here, we investigated the molecular consequences of oxygen concentration on 28-day ex vivo HSC cultures using single cell RNA-seq Overall design: We examined fresh c-Kit+ bone marrow and day-28 cultured HSCs incubated at 20% oxygen, 5% oxygen, or 1% oxygen