CsGOGAT Is Important in Dynamic Changes of Theanine Content in Postharvest Tea Plant Leaves under Different Temperature and Shading Spreadings

We
analyzed the changes of theanine content in postharvest tea
leaves under high temperature (38 °C), low temperature (4 °C),
and shading spreadings by using ultrahigh-performance liquid chromatography.
The differentially expressed proteins (DEPs), CsFd-GOGAT and CsNADH-GOGAT,
which are involved in theanine biosynthesis pathway, were identified
from the corresponding proteome data. The protein–protein interactions
of CsFd-GOGAT and CsNADH-GOGAT, CsTS1, or CsNiR were verified by yeast
two-hybrid technology. The expression profiles of 17 genes in theanine
metabolism, including CsFd-GOGAT and CsNADH-GOGAT, were analyzed by quantitative real-time polymerase chain reaction.
The correlations between the dynamic changes of theanine content and
expression profiles of related genes and DEPs were analyzed. This
study preliminarily proved the importance of CsGOGAT in dynamic changes
of theanine content in postharvest tea leaves during spreading.