A herpesvirus microRNA targets host microRNA processing to promote virus reactivation from latency [dRNA-seq]

In a major study, we found that miR-aU14, a potential miRNA expressed by human herpesvirus 6A (HHV-6A), acts to selectively inhibit the processing of members of the human miR-30 family through direct RNA:RNA interaction and causes changes in mitochondrial arcchitecture through p53-Drp1 axis. In order to characterize miR-aU14, we carried out differentia lRNA-seq (dRNA-seq) from HHV-6A lytic infected HSB2 cells. Overall design: HSB-2 cells were obtained from HHV-6 Foundation, USA and were maintained in RPMI 1640 media with 200 units of Penicilin and Streptomycin. HSB2 cells were infected with HHV-6A at an MOI of 5-10. 3 days post infection, total RNA was prepared using TRI-reagent (SIGMA). Two biological replicates were prepared on two different days. Transcription start site profiling dataset using dRNA-seq was prepared according to the published protocol (Sharma and Vogel, 2014) with some modifications by the Core Unit Systems Medicine (Würzburg). In brief, total RNA was extracted from HHV-6A and mock infected HSB-2 cells.