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ddRAD sequencing data of tomato genotypes

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NIAID Data Ecosystem2026-05-02 收录
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The genomic data of two tomato genotypes (E42 and LA3120) were obtained by using ddRAD sequencing technology. Total genomic DNA was extracted from 100 mg of young leaf tissue from the two genotypes using the DNeasy plant mini kit (Qiagen, Hilden, Germany). For DNA sequencing, samples were used to prepare libraries for the ddRAD sequencing, as described in Peterson et al. 2012, PLoS ONE 7, e37135 with minor modifications. MboI and SphI enzymes were used for restriction digestion and fragments sequenced with the V4 chemistry paired end 125 bp mode on the HiSeq2500 instrument (Illumina, San Diego, CA, USA).
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2024-07-11
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