Comparison of NAT-lncRNA knockout effect by next-generation sequencing

Analysis of different expression after lncRNA DHRS4-AS1 knockout with two strategies in immortalized human pancreatic duct epithelial cell line HPDE6-C7. The strategies include promoter-exon1 (PE) and branch point and 3‘ splicing site (BESST). The results provide transcriptome comparison between PE and BESST knockout approach, indicating the superior effect of BESST. The HPDE-C7 cell line was used in this study. CRISPR-based PE and BESST were applied to knockout DHRS4-RS1 lncRNA. Triplicates were done for each group.