Euphausiid-specific metabarcoding data from eDNA samples collected on the TEMPO voyage

This is a Euphausiid-specific metabarcoding dataset of Southern Ocean eDNA samples collected on the TEMPO voyage in 2021. We collected eDNA samples by filtering five litres of seawater in the East Antarctic Southern Ocean from the surface and seafloor as well as in and near krill swarms(n = 110). After DNA extraction, a Euphausiid-specific metabarcoding marker was used to determine the presence of different Southern Ocean krill species in each sample.
Detailed methods are available in the following publication:
Suter L et al. (2025) Monitoring Antarctic krill (Euphausia superba) distribution in the Southern Ocean: environmental DNA (eDNA) adds to the toolbox. Frontiers in Marine Science.
The data provided here contains
- raw paired end sequencing data in fastq format for each sample
- R markdown code to process the raw data in order to identify unique sequences, and which sequences are contained in which samples
- a tag file that identifies which multiplex-identifier (MID) tags were used in forward and reverse primers for each sample
- a zOTU table that identifies which sequences were detected in each sample, and what taxonomy was associated with each sequence.