Impact of decreasing RNA input amounts on overall probe sets detected at varying transcript abundances by the WT-Ovation FFPE and WT-Ovation One-Direct RNA amplification systems.

The number of probe sets detected at each signal intensity level (as a measure of transcript abundance) is shown. Numbers are the mean of duplicate arrays for input of 500 pg and below. Reduction of input RNA amounts has a minimal impact on the overall numbers of probe sets detected, but which is more evident with the One-Direct system, with a greater number of more abundant transcripts not being detected with decreasing RNA input.