Figure S1 - Alternative mRNA Splicing Generates Two Distinct ADAM12 Prodomain Variants
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https://figshare.com/articles/dataset/_Alternative_mRNA_Splicing_Generates_Two_Distinct_ADAM12_Prodomain_Variants_/816467
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The anti-ADAM12 antibody (R&D Biosciences; clone 632525) used for flow cytometry recognizes both ADAM12-La and ADAM12-Lb. MCF10A cells stably transduced to express ADAM12-La or ADAM12-Lb were subjected to immunoprecipitation using mouse anti-ADAM12 antibody, clone 632525, and Protein G agarose. IgG isotype control was used as a negative control. Equal volumes of total cell lysates of ADAM12-La- and ADAM-Lb-expressing cells (Inputs), as well as equal volumes of Protein G eluates, were analyzed by Western blotting using rabbit polyclonal anti-ADAM12 antibody #3394.
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创建时间：
2013-10-07
