Recruitment Tbk1 to dsDNA:Dai followed by its activation

In murine L929 cells Dai dimer formation enables recruitment of Tbk1 and Irf3 to the C-terminal region of Dai in response to cytosolic DNA. This interaction is DNA-dependent as Dai mutants that lack DNA binding domains neither recruited Tbk1 nor activated Irf3 (Takaoka A et al 2007).

在鼠源L929细胞中，Dai二聚体的形成能够介导Tbk1和Irf3蛋白向Dai蛋白C端结构域的募集，以响应细胞质DNA的存在。此相互作用依赖于DNA结合域，因为缺乏DNA结合域的Dai突变体无法募集Tbk1或激活Irf3（Takaoka A 等，2007年）。