A collection of 3D-SIM images of telomeres, centromeres and nuclei in HeLa cells

This collection contains several datasets of 3D-SIM images of nuclei in HeLa cells. Nuclei were counter-stained with Hoechst. Immunolabelling was used to target nuclear envelope (SUN-1), nuclear lamina (LaminAC) and either telomeres (TRF1-GFP, labeled with GFP-nanobodies) or centromeres (CREST). For telomeres, there were two experimental conditions: untreated cells and cells treated by siRNA against BAF and LAP2. SUN-1 labelling was used to identify early G1 stages. For siRNA experiments, where no cell synchronization could be performed, the centrosomal protein Cep152 was added in the same channel as SUN-1 to identify cell cycle stages.